BlastP

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Tutorial for simple Blastp search for finding human homologs

Blastp is the most frequently used method for searching a protein sequence database for similar sequences. The algorithm for Blast will be dealt with in detail in the course MOL204 (Applied Bioinformatics I). Here you are asked to run the program with your sequence and with the search parameters given here in the tutorial. Feel free, however, to explore the program with other sequences and search parameters.

Consult the NCBI Blast home page for more information, documentation and help.

  1. Go to the NCBI Blast search page (Make sure that the blastp tab is selected in the tab heading).
  2. Copy the fasta version of your protein sequence into the window under "Enter Query Sequence"
  3. From the "Database" pull-down tab under "Choose Search Set", select "UniProtKB/Swiss-Prot
  4. In the "Organisms" window, type "homo sapiens" and select Homo sapiens (taxid:9606) when it appears.